The new cells were mostly built in the morning (from 2:00 to 10:00 am) and the queen laid eggs (2.52 ± 0.81 sec per egg) and capped the cells in the afternoon (from 12:00 to 17:00 pm). The worker cell dimensions were 5.30 ± 0.34mm in length by 4.28 ± 0.37mm in width with a volume of 45.02 ± 5.74mL (food volume: 19.05 ± 3.5mL ratio: 42.27%). In this study, the biological characteristics of Tetrigonilla collina, as a representative for Southeast Asian stingless bees, were observed and recorded. Sixteen species of stingless bees have been identified in Vietnam (Sakagami, 1975 Sakagami, 1978 Engel, 2000 Chinh et al., 2005 Rasmussen, 2008). There are over 16,000 species of stingless bees around the world, among which, 42 species are from Asia (Kerr & Maule, 1964). Stingless bees are distributed in tropical and subtropical regions.
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